Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is seeking a highly motivated, collaborative, and innovative postdoctoral scientist with a background in antibody discovery and engineering to join Entrada’s discovery efforts. The candidate will work with world-class, multidisciplinary team of drug discovery scientists on applying cutting-edge delivery technology to develop leads for compelling intracellular biological targets. Specifically, the candidate will design and execute biologics against therapeutically relevant intracellular targets. The candidate will contribute to scientific publications and regulatory submissions as required.  

Job Responsibilities

• Design and execute antibody library screening against novel therapeutic targets, working closely with CRO’s as needed 
•  Develop and execute display techniques, flow cytometry, HTS for the generation and optimization of novel biotherapeutics 
• Develop and execute assays for screening leads (e.g. ELISA, Octet, and SPR) and cell based potency assays 
•  Develop protein conjugations and optimize PK/PD * Expertise executing antibody developability assays and formulation 
• Champion innovation and new initiatives in discovery programs by proactively reviewing literature and other advancements 
•  Interpret and present research data and findings in internal and external scientific meetings and conference
• Contribute effectively to patents, reports, and publications of scientific findings 

Qualifications

•  Ph.D. in Biological Science (Molecular Biology, Structural Biology, Protein Biochemistry, Cell Biology) or a related field Skills and Experiences 
• Experience in antibody development including single domain antibody formats and engineering such as antibody humanization, CDR grafting, biophysical properties optimization, and protein structural modeling 
• Experience working with biophysical characterizations of biotherapeutics, such as SPR, Octet, and DSC 
• Experience displaying techniques such as phage/yeast display is highly desired
•  Experience with protein conjugation and/or antibody drug conjugates is highly desired 
• Strong motivation, passion and commitment to science and engineering of novel entities 
• Strong time management skills and ability to manage multiple projects in parallel
• Ability and desire to work in an interdisciplinary scientific team and the desire to learn new techniques 
• Ability to work with junior scientists to facilitate troubleshooting activities is desired

Compensation is competitive and commensurate with experience. Competitive health, dental and vision coverage. Life insurance and short-term and long-term disability insurance provided. Free monthly subway pass, subsidized parking or subsidized monthly commuter rail pass. 

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for an independently motivated and detail-oriented Senior Research Associate with excellent problem-solving abilities for interrogating complex molecular entities. In this largely laboratory-based position, the Senior Research Associate will focus on providing analytical support for Entrada’s intracellular biologics Process Development team. The successful candidate will design and execute a range of chromatography-based analytical methods as well as biochemical and biophysical assays to support bioanalytical characterizations during the discovery and development phases. The successful candidate will also contribute to tech transfer to CDMOs as required.

Job Responsibilities

• Execute analytical and biophysical assays to support the process development and drug release of intracellular biologics 
•  Execute release and characterization assays and facilitate assay transfers to CDMOs 
•  Prepare and present technical data, technical reports and standard operating procedures (SOPs) for internal use and transfer to external sites 
•  Work independently and collaboratively in a scientifically-stimulating, patient-focused research environment  

Qualifications

• A Bachelor’s in biochemistry, chemistry, biophysics, biochemistry or a related discipline with 5+ years industry experience or a Master’s with 3+ years industry experience 
• HPLC/UPLC experience with a fundamental understanding of liquid chromatographic methods (RP, SEC, IEX, HIC) is required
• Experience with characterizing proteins, antibodies and/or other biologics by analytical methods such ELISA, PAGE, capillary electrophoresis and western blotting is required 
•  Hands-on experience with biophysical characterization techniques, including Octet and MALS/DLS preferred 
• Hands-on experience with bioburden and host cell protein assays preferred 
• A basic understanding of drug substance manufacturing principles and unit operations • A strong work ethic and excellent time management skills  

Compensation is competitive and commensurate with experience. Competitive health, dental and vision coverage. Life insurance and short-term and long-term disability insurance provided. Free monthly subway pass, subsidized parking or subsidized monthly commuter rail pass. 

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for a highly motivated, collaborative and innovative Principal Scientist, specializing in pharmacokinetics and pharmacology to join the Company’s intracellular biologics discovery and development efforts. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists and lead the in vitro and in vivo ADME, PK, PD and toxicokinetic efforts. Specifically, the candidate will design, manage and execute in vitro and in vivo programs both inhouse and at contract research organizations. The candidate will also be responsible for analyzing, interpreting and modeling DMPK and PK/PD data. The candidate will contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Design, manage and execute in vitro, and in vivo experiments to evaluate the ADME and PK profiles as well as toxicokinetics and immunogenicity of developmental intracellular biologics candidates both in-house and at contract research organizations 
•  Develop and execute assays for measuring drug concentration, target engagement and pharmacodynamic effects in vitro and in vivo 
• Champion innovation and new initiatives for discovery programs by proactively reviewing literature and other advancements in disease area biology 
• Interpret and present research data and findings in internal and external scientific meetings and conferences
•  Contribute effectively to patents, reports and publications of scientific findings

Qualifications

• Ph.D. in pharmaceutics, pharmacology or a relevant scientific discipline • A minimum of 8 years’ experience in pharmacokinetics and pharmacology 
• Demonstrated knowledge of ADME, PK/PD and bioanalytical development of protein therapeutics/biologics 
• Deep understanding of translational biology
• Experience in bioanalytical techniques including assays for immunogenicity, in vitro and in vivo toxicology and histopathology preferred
• Experience in assay development for target engagement and biomarker discovery preferred Experience in contributing DMPK and pharmacology sections of regulatory submissions preferred
•  Ability to collaborate in an interdisciplinary team setting with excellent written and oral communication skills 
•  Ability to multi-task to meet research and development goals
•  Drive to solve problems and ability to work independently Compensation is competitive and commensurate with experience. 

Competitive health, dental and vision coverage. Life insurance and short-term and long-term disability insurance provided. Free monthly subway pass, subsidized parking or subsidized monthly commuter rail pass. 

Company Overview

Position Summary

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol. Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Job Responsibilities

• Performing in vitro assays using mammalian cell lines according to SOPs, troubleshooting as needed 
• Planning and performing of all technical aspects of in vivo experiments 
• Rodent maintenance, dosing, sampling, necropsy and tissue/organ dissection
• Maintaining careful laboratory notebooks and generating high quality study reports. 
• Presenting data and research findings in internal team meetings

Qualifications

• A Bachelor's or Master’s degree in biology, cell biology or a related field with at least 3 years of laboratory experience is required. Preference for industry or startup R&D experience
• Demonstrated understanding of cell biology and hands-on in vitro research experience in techniques including cell-based functional assays, cell viability, fluorescence imaging and biochemical assays such as western blotting and qPCR 
• Experience with in vivo PK/PD studies in rodents, including various modes of pharmacological agent administration and blood collection, in addition to experience with tissue/cell isolation for biological analysis 
• Strong communication skills (oral and written) and ability to work effectively across functions 
• Desire to work in an innovative and collaborative environment pursuing high reward science

Compensation is competitive and commensurate with experience. Competitive health, dental and vision coverage provided, and other benefits.

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is seeking a highly motivated, collaborative, and innovative Research Associate with a background in protein production, purification and characterization to join Entrada’s discovery efforts. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists to express multiple therapeutic proteins and enzymes for evaluation as new clinical candidates. Specifically, the candidate will design protein expression constructs, develop and optimize protein purification processes, characterize purified material using standard biochemical techniques and develop enzymatic activity assays. The candidate will assist with assay development and metabolite analysis, as well as evaluate new lab equipment and vendors, and engage with external contract research organizations as needed. The candidate will also contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Optimize production and FPLC-based purification of recombinant proteins and enzymes using microbial expression systems
• Characterize proteins using PAGE, Western Blot, RP-HPLC, SEC, LAL, UV-Vis spectroscopy, mass spectrometry, host cell-DNA, host protein, etc.
• Develop and execute enzymatic activity assays
• Prepare SOPs and other reports/documents for assay development and validation
• Interpret and present research data and findings in internal scientific meetings
• Contribute effectively to patents, reports and publications of scientific findings

Qualifications

• Bachelor’s and/or Master’s degree in biochemistry, biophysics, pharmacology, biomedical engineering or a relevant scientific discipline and a 2+ years’ experience in protein expression, purification and analytics.
• Use of biochemistry laboratory techniques
• Use of molecular biology techniques
• Recombinant protein production from microbial systems (eg. E. coli and Pichia pastoris)
• Protein purification both from soluble fraction and inclusion bodies with AKTA systems, using affinity, size exclusion, ion exchange and other chromatography
• Knowledge of enzymology, enzyme kinetics and enzyme assay development a plus
• Ability to collaborate with interdisciplinary team with excellent written and oral communication skills
• Ability to multi-task to meet research and development goals
• Drive to solve problems and ability to work independently