Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for a highly motivated, collaborative, and innovative Cellular Pharmacology, Research Associate Scientist in support of the Company’s intracellular oligonucleotide and biologics discovery and development efforts. This individual will work closely with the Discovery Group and other functions to progress programs from target identification to preclinical proof-of-concept. The successful candidate is expected to be involved in all aspects of molecular cellular pharmacology needs, leading efforts for developing cell-based assays for candidate optimization, drug candidate validation and overseeing in vitro pharmacology studies for preclinical proof-of-concept. The successful candidate will also provide biology insights on new targets This individual is expected to work with Scientist/Sr Scientist in Pharmacology group to perform experiments and biological research. As part of the Pharmacology Group, the Research Associate has the opportunity to contribute broadly in advancing validated targets into the drug discovery pipeline.

Job Responsibilities

• Responsible for the integrity and quality of internal in vitro data package
• Design, conduct and manage in vitro biologically relevant cellular assays to evaluate mechanistic and physiologically relevant functional activity data package.
• Collaborate with Discovery Group to generate mechanistic proof-of-concept and contribute to selecting novel therapeutic modalities that includes antibody, peptides, and oligonucleotides
• Utilize internal and external resources to execute pharmacology studies and preclinical translational studies relevant for rare neuromuscular and immunology related disease
• Independently design molecular or cellular assays aligned with the targeted disease mechanism
• Present data and research findings in the weekly pharmacology meeting including cross functional discovery/antibody meetings

Qualifications

• BS/MS, with 2+ years in academic environment, industry experience preferred
• Experience in state-of-the-art in vitro laboratory techniques (PCR, DNA and protein gel electrophoresis, Western blots, RNA preps, quantitative real-time PCR, mammalian cell line tissue culture.
• Exposure to mammalian transfection and lentiviral related work experience preferred not mandatory
• Demonstrated ability to design and perform experiments, assays is necessary but ability to troubleshoot and solve problem is preferred
• Able to solve routine operation and scientific problems requiring original thinking and deductive reasoning, nice to have
• Must have good working knowledge and familiarity to Microsoft Office (Word, Excel, PowerPoint), Graphic software is required
• Strong verbal and written communication skills is a must
• Ability to work effectively across cross functional teams is required, not mandatory
• “Hands-on” mentality in moving projects forward is preferred

The Perks

• Competitive health, dental, and vision coverage
• Life insurance, short term, long term disability insurance provided
• Unlimited Vacation
• 401K
• Free subway and local bus use, subsidized parking, or subsidized monthly commuter rail pass

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

To learn more or submit your resume, contact us at careers@entradatx.com.

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics’ development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for an in Vivo Pharmacology Research Associate Scientist to drive the Company’s intracellular biologics discovery programs. The successful candidate will work closely with the highly engaged, world-class, multidisciplinary team of drug discovery scientist and other functions to progress programs from target identification to preclinical proof-of-concept studies. The successful candidate is expected to be involved in all aspects of pre-clinical in vivo studies and overseeing execution of pharmacology studies for dosing, tissue collection and data analysis. This individual is expected to directly work with Scientist/Sr Scientist in Pharmacology group for execution and completion of some of the pre-clinical PoC studies. As part of the Pharmacology Group, the in vivo pharmacology role could contribute broadly advancing validated targets into the drug discovery portfolio pipeline.

Job Responsibilities

• Demonstrated experience with independently running and analyzing data from in vivo studies, for example mouse disease models including genetic neuromuscular/xenograft/syngeneic mouse models
• Required skills include proficiency in dosing (IV, IP, IT, SC, I-TUMOR and PO routes), blood collection and tissue/tumor collection
• Experience with monitoring solid tumor growth in mouse models and tumor model development, familiarity working with GEMM mouse model for rare disease indications
• Demonstrated ability to design, conduct, and analyze data from in vivo pharmacology studies
• Experience in vitro cell culture, compound preparation/formulation, and administration
• Present data and research findings in internal team meetings, review meetings and external conferences

Qualifications

• BS or equivalent in biology or related field with 3-4 years of in vivo experience
• Experience in rare genetic related disease indication is preferred, in addition to proficiency in Oncology/immunology related model development
• Hands on experience with ex vivo techniques such as qPCR, RT-PCR and western blot analysis is highly desired, familiarity with flow cytometry is preferred but not mandatory
• Excellent verbal and written communication skills and the ability to work effectively across functions
• Desire to work in an innovative and collaborative environment pursuing high reward science

The Perks

• Competitive health, dental, and vision coverage
• Life insurance, short term, long term disability insurance provided
• Unlimited Vacation
• 401K
• Free subway and local bus use, subsidized parking, or subsidized monthly commuter rail pass

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

To learn more or submit your resume, contact us at careers@entradatx.com.

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of oligonucleotide therapeutics, proteins, and peptides, thus allowing for the development of programs across several intracellular target classes. The company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is seeking a highly motivated, collaborative, and innovative Principal Scientist specializing in oligonucleotide chemistry to build a state-of-the art synthesis and purification lab. The candidate will work with a world‐class, multidisciplinary team of drug discovery and development scientists on applying Entrada’s cutting‐edge drug delivery platform to develop oligonucleotide‐based pipelines for treating some of the most challenging diseases. Specifically, the candidate is expected to start the synthesis, purification, and characterizations of oligonucleotides. The successful candidate will also recruit and build as well as lead a team of excellent scientists and associates.

Job Responsibilities

• Establish oligo synthesis lab to enable milligram to gram synthesis of oligonucleotides and their conjugates
• Establish purification and analysis methods
• Recruit and build an oligonucleotide chemistry team
• Manage the supply of raw material and external CRO
• Manage necessary equipment and training as well as mentor junior members
• Interact with external contract organizations to manage orders, assist with timeline, quality control, trouble shooting, and facilitating method transfer as needed
• Interpret and present research data and findings in internal and external scientific meetings and conferences
• Contribute effectively to patents, reports, and publications of scientific findings

Qualifications

Education Requirements

Ph.D. in organic chemistry with a minimum of 8 years’ experience in oligonucleotide chemistry experience

Skills and Experiences

• Demonstrated experience in oligonucleotide chemistry including synthesis, purification, and analysis
• Demonstrated experience in nucleotide chemistry
• Hands on experience with oligonucleotide synthesizers as well as other synthesis and purification equipment
• Previous experience in supervising, developing, and recruiting excellent scientists
• Strong track record of publication and presentation in oligonucleotide therapeutics field
• Ability to collaborate with interdisciplinary team with strong written and oral communication skills
• Ability to multi‐task to meet research and development goals

The Perks

• Competitive health, dental, and vision coverage
• Life insurance, short term, long term disability insurance provided
• Unlimited Vacation
• 401K
• Free subway and local bus use, subsidized parking, or subsidized monthly commuter rail pass

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

To learn more or submit your resume, contact us at careers@entradatx.com.

Company Overview

Entrada Therapeutics’ mission is to treat devastating diseases through the intracellular delivery of biologics.

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is seeking a highly motivated, collaborative and innovative Research associate specializing in oligonucleotide therapeutics, including but not limited to antisense oligonucleotides and small interfering ribonucleic acids. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists on applying Entrada’s cutting-edge drug delivery platform to develop oligonucleotide-based pipelines for treating some of the most challenging diseases. Specifically, the candidate will work together with scientists to design, prepare and characterize oligonucleotide constructs, and carry out analytical assays for functional validation both in vitro and in vivo. The candidate will contribute to reports and scientific publications as required.

Job Responsibilities

• Process and analyze samples from in vitro and in vivo studies (e.g. RNA/protein extraction, RT-PCR, RT-qPCR, ELISA, Western blotting, confocal microscopy)
• Prepare and QC oligonucleotides-peptide conjugates for in vitro and in vivo study
• Culture and maintain cell lines for in vitro study
• Develop and execute cell-based assays for validating biological activity of oligonucleotides-peptide conjugates in vitro
• Prepare SOPs and other reports/documents for assay development and validation
• Contribute to patents, reports, and publications

Qualifications

Education Requirements

Bachelor or Master in Chemistry, Biochemistry, or related field with 2-year lab experience

Skills and Experiences

• Hands-on biochemistry experience, such as RT-PCR, qPCR, ELISA, fluorescence microscopy and Western blotting
• Hands-on experience in oligonucleotides, such as antisense oligonucleotides and siRNAs
• Hands-on experience in cell culture
• Ability to collaborate with interdisciplinary team with strong communication skills
• Desire to work in an innovative and collaborative environment pursuing high reward science
• Desire to work in an innovative and collaborative environment pursuing high reward science

The Perks

• Competitive health, dental, and vision coverage
• Life insurance, short term, long term disability insurance provided
• Unlimited Vacation
• 401K
• Transportation stipend

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

To learn more or submit your resume, contact us at careers@entradatx.com.

Education Requirements

Ph.D. in biochemistry, biology, or related field with 3+ years post-degree experience

Skills and Experiences

• Demonstrated experience in designing and screening of oligonucleotides in vitro using patient-derived cells or constructed cellular model
• Demonstrated experience in cell‐based screening assays, such as qPCR, ELISA, nanoluciferase, fluorescence and Western blotting
• Experience in designing effective splice-switching antisense oligonucleotides for exon skipping and exon inclusion is preferred
• Expertise in cell culture including working with complex cell types preferred
• Ability to collaborate with interdisciplinary team with excellent written and oral communication skills
• Ability to multi‐task to meet research and development goals
• Drive to solve problems and ability to work independently

Position Summary

Entrada is seeking a highly motivated, proactive, collaborative, and innovative scientist with a background in bioorganic chemistry to join Entrada’s discovery efforts. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists on applying cutting-edge peptide and oligonucleotide chemistry to develop leads for compelling biological targets. Specifically, the candidate will design and execute medicinal chemistry studies and use proprietary display technology to develop next-generation constrained peptidomimetics. The candidate will contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Design and execute the synthesis, purification, and characterizations of peptides and oligonucleotides development candidates
• Design and execute the synthesis and characterizations of peptide and oligonucleotide library for SAR studies
• Work with other team members to implement assays for ascertaining target engagement, validating biological activity in vitro (e.g. alphascreen, TR-FRET, fluorescence polarization, cell-based functional assay)
• Manage, organize, and critically evaluate data independently.
• Champion innovation and new initiatives in discovery programs by proactively reviewing literature and other advancements
• Interpret and present research data and findings in internal and external scientific meetings and conferences
• Contribute effectively to patents, reports, and publications of scientific findings

Education Requirements

• Ph.D. in organic chemistry, chemical biology, medicinal chemistry, bioorganic chemistry, or a relevant scientific discipline

Skills and Experiences

• Demonstrated experience in the design, synthesis, and purification of peptides and oligonucleotides
• Skilled with solid-phase peptide synthesizers, method optimization and conjugations
• Demonstrated experience with HPLC, LC-MS systems and other analytical instruments
• Hands-on experience with oligonucleotide synthesizers is a plus
• Hands-on experience with flow-based solid phase synthesis is a plus
• Experience with hit optimization, SAR study, target engagement and other medicinal chemistry principals is a plus
• Ability to collaborate with interdisciplinary team with excellent written and oral communication skills
• Ability to learn fast, grasp the “essence”, and multi-task to meet research and development goals
• Drive to solve problems and ability to work independently

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

We currently have an exciting opportunity available to join the Upstream (cell culture/fermentation) group at Entrada Therapeutics. We are seeking a highly motivated, collaborative, and innovative Sr. Research Associate with a background in fermentation or/and cell culture. The candidate will be responsible for the designing and execution of small- and medium-scale mammalian cell culture and microbial fermentations to support the discovery and development of Entrada’s biologics pipeline. The candidate will work with a world-class, multidisciplinary team of drug discovery and development scientists to evaluate new clinical candidates and develop new processes. Specifically, the candidate will focus on the improvements of upstream and high throughput screening workflow to produce recombinant therapeutic proteins using microbial and mammalian expression systems. The candidate will also contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Plan the cell culture, fermentation and primary recovery experiments according to objective priority by interacting with various departments within Entrada
• Independently designs and troubleshoots fermentation, cell culture and primary recovery experiments
• Set-up, inoculate, and operate meticulously shake flasks, small- and medium-scale mammalian cell culture, yeast and bacterial fermentations
• Perform primary recovery such as cell harvest, depth filtration, homogenization, inclusion body wash, solubilization, and refolding
• Run SDS-PAGE gels, HPLC, Cedex Bio to monitor cell growth, metabolite levels and protein expression
• Record experimental details and observations in the laboratory notebooks
• Present data and research findings in internal team meetings

Qualifications

• Ph.D./MS/BS in Biochemical Engineering, Biotechnology, Biochemistry or related fields
• Experience working with E. coli, Pichia pastoris, or CHO for expression of recombinant proteins
• Experience with primary recovery operations such as centrifugation, depth filtration, homogenization, solubilization, refolding (a plus)
• Knowledge and use of molecular biology and biochemistry techniques
• Strong understanding of bioreactor design and transport phenomena as it relates to bioprocess scale-up and applied control theory
• Desire to work in an innovative and collaborative environment pursuing high reward science
• Ph.D. or MS with 2+ years or BS with 4+ years of experience related to cell culture or fermentation process development of recombinant proteins

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics’ development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for a Principal Scientist focusing on Neuromuscular biology to drive the Company’s intracellular Oligo/biologics discovery programs. The successful candidate will work closely with the Discovery Group and other functions to progress programs from target identification to preclinical proof-of-concept. The successful candidate is expected to be involved in all aspects of pre-clinical pharmacology studies, leading efforts for developing GEMM for neuromuscular disease indication, functional physiological measurement of cell-based assays for providing mechanistic insight, drug candidate validation. The successful candidate will also provide muscle biology insights on neuromuscular targets and therapeutic hypotheses to guide drug discovery efforts and translation into the clinic. This individual is expected to directly lead from the bench and work with research associates and CROs to perform experiments and biological research. As part of the Pharmacology Group, this role has the opportunity to contribute broadly to advancing validated targets into the drug discovery pipeline.

Job Responsibilities

• Design and lead the preclinical studies to advance lead programs in Neuromuscular rare disease space
• Lead target nomination, analysis and building scientific rationale for disease selection
• Evaluate, validate,and develop disease models for neuromuscular diseases
• Investigate and validate efficacy of oligonucleotide mediated delivery by WB, RT-PCR, IF, and other tools both in vivo and in vitro to drive go/no-go decisions
• Manage in house vivarium operations by working with junior associates and scientists
• Collaborate with Discovery Group to generate mechanistic proof-of-concept and contribute to selecting novel therapeutic modalities that include oligonucleotides, peptides, and antibodies
• Utilize internal and external resources to execute pharmacology studies and preclinical translational studies relevant for neurological disorders
• Present data and research findings in internal team meetings, review meetings and external conferences

Qualifications

• Ph.D. or equivalent in neuromuscular biology or relevant field with strong publication record with 6+ industry experience
• Experience in performing in vitro and in vivo efficacy studies with GEMM for neuromuscular indications
• Experience in muscle physiological/functional measurement
• Experience in technologies including ELISA, immunohistochemistry, immunofluorescence, in-situ hybridization, western blotting, flow cytometry and qPCR
• Proven ability to work independently, solve problems and initiate new ideas
• Strong communication skill and ability to work effectively across functions
• “Hands-on” mentality in moving projects forward
• Desire to work in an innovative and collaborative environment pursuing high reward science

The Perks

• Competitive health, dental, and vision coverage
• Life insurance, short term, long term disability insurance provided
• Unlimited Vacation
• 401K
• Free subway and local bus use, subsidized parking, or subsidized monthly commuter rail pass

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

To learn more or submit your resume, contact us at careers@entradatx.com.

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of macromolecules including oligonucleotides and biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the intracellular delivery of therapeutics.

Entrada is comprised of experts and leaders in both drug development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring a highly motivated, collaborative and innovative Drug Metabolism and Pharmacokinetics (DMPK) expert to lead our preclinical DMPK activities in support of the Company’s intracellular oligonucleotide and biologics discovery and development efforts. The successful candidate will report to the Head of Product Development and work with a world-class, multidisciplinary team of drug development scientists and lead the design, analysis and interpretation of preclinical in vitro and in vivo studies. Specifically, the candidate will design, manage and execute in vitro and in vivo programs both in-house and at contract research organizations. The candidate will also be responsible for analyzing, interpreting and modeling DMPK and PK/PD studies. The successful candidate will also have exceptional verbal and written communication skills and will be expected to contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Lead all aspects of DMPK design, manage and execute in vitro/in vivo experiments to support preclinical characterization of drug candidates
• Develop and coordinate the execution of studies for measuring drug concentration, target engagement and pharmacodynamic effects in vitro and in vivo
• Lead and coordinate outsourced preclinical studies by interacting closely with CROs
• Partner with the discovery team, toxicology and clinical managers to optimize preclinical models and lead candidates to inform first-in-patient clinical dosing strategies
• Author study reports to support IND filings
• Champion innovation and new initiatives for discovery programs by proactively reviewing literature and other advancements in disease area biology
• Interpret and present research data and findings in internal and external scientific meetings and conferences
• Contribute effectively to patents and scientific publications

Qualifications

Required

• Ph.D. in pharmacology or a relevant scientific discipline
• 6+ years of relevant industrial or academic experience in pharmacokinetics and pharmacology
• Demonstrated knowledge of ADME, PK/PD, toxicokinetics and bioanalytical development of protein therapeutics/biologics/oligonucelotides
• Deep understanding of translational biology
• Significant experience in bioanalytical techniques including assays for assessing serum/tissue drug levels and immunogenicity
• Experience in pre-clinical PK-PD modeling
• Experience with writing technical study reports and supporting IND submissions
• Ability to collaborate in an interdisciplinary team setting spanning discovery to clinical operations and strategy with excellent written and oral communication skills
• Ability to manage multiple high priority activities to support research and company development goals
• Excellent analytical reasoning skills and the ability to effectively troubleshoot and perform root cause analyses

Preferred

• Understanding of in vitro and in vivo toxicology assessment and histopathology
• Experience in assay development for target engagement and biomarker discovery
• Understanding of clinical trial design and PK modeling to support first-in-human clinical studies