Company Overview

Entrada Therapeutics’ mission is to treat devastating diseases through the intracellular delivery of biologics.

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is seeking a highly motivated, collaborative and innovative Research associate specializing in oligonucleotide therapeutics, including but not limited to antisense oligonucleotides and small interfering ribonucleic acids. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists on applying Entrada’s cutting-edge drug delivery platform to develop oligonucleotide-based pipelines for treating some of the most challenging diseases. Specifically, the candidate will work together with scientists to design, prepare and characterize oligonucleotide constructs, and carry out analytical assays for functional validation both in vitro and in vivo. The candidate will contribute to reports and scientific publications as required.

Job Responsibilities

• Process and analyze samples from in vitro and in vivo studies (e.g. RNA/protein extraction, RT-PCR, RT-qPCR, ELISA, Western blotting, confocal microscopy)
• Prepare and QC oligonucleotides-peptide conjugates for in vitro and in vivo study
• Culture and maintain cell lines for in vitro study
• Develop and execute cell-based assays for validating biological activity of oligonucleotides-peptide conjugates in vitro
• Prepare SOPs and other reports/documents for assay development and validation
• Contribute to patents, reports, and publications

Qualifications

Education Requirements

Bachelor or Master in Chemistry, Biochemistry, or related field with 2-year lab experience

Skills and Experiences

• Hands-on biochemistry experience, such as RT-PCR, qPCR, ELISA, fluorescence microscopy and Western blotting
• Hands-on experience in oligonucleotides, such as antisense oligonucleotides and siRNAs
• Hands-on experience in cell culture
• Ability to collaborate with interdisciplinary team with strong communication skills
• Desire to work in an innovative and collaborative environment pursuing high reward science
• Desire to work in an innovative and collaborative environment pursuing high reward science

Benefits

• Competitive health, dental, and vision coverage
• Life insurance, short term, long term disability insurance provided
• Unlimited Vacation
• 401K
• Transportation stipend

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

To learn more or submit your resume, contact us at careers@entradatx.com.

Education Requirements

Ph.D. in biochemistry, biology, or related field with 3+ years post-degree experience

Skills and Experiences

• Demonstrated experience in designing and screening of oligonucleotides in vitro using patient-derived cells or constructed cellular model
• Demonstrated experience in cell‐based screening assays, such as qPCR, ELISA, nanoluciferase, fluorescence and Western blotting
• Experience in designing effective splice-switching antisense oligonucleotides for exon skipping and exon inclusion is preferred
• Expertise in cell culture including working with complex cell types preferred
• Ability to collaborate with interdisciplinary team with excellent written and oral communication skills
• Ability to multi‐task to meet research and development goals
• Drive to solve problems and ability to work independently

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of oligonucleotide, proteins, and peptides, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cell. Entrada is comprised of experts and leaders in both biologics development and the rare disease space.

Position Summary

Entrada is seeking a highly motivated, collaborative and innovative Scientist/Sr. Scientist specializing in oligonucleotide chemistry, including but not limited to the design, synthesis, purification, and analysis of oligonucleotides, linkers, and protein/peptide/antibody conjugates. The candidate will work with a world‐class, multidisciplinary team of drug discovery scientists on applying Entrada’s cutting‐ edge drug delivery platform to develop oligonucleotide-based pipelines for treating some of the most challenging diseases. Specifically, the candidate will start the oligonucleotide synthesis at Entrada including establishing the oligo synthesis lab and setting up synthesis, purification, and analytical method protocols. The candidate will mentor junior associates and contribute to the development of drug delivery platform with an interdisciplinary team.

Job Responsibilities

• Establish oligo synthesis lab to enable milligram to gram scale synthesis andpurification of oligonucleotides
• Design and optimize oligonucleotide sequences for discovery programs
• Develop and execute the purification, analytical methods for oligonucleotides and its conjugates with peptide and proteins
• Manage outsourcing chemistry activities to accelerate internal research activities
• Prepare SOPs and other reports/documents for assay development and validation
• Interpret and present research data and findings in internal and external scientific meetings and conferences
• Contribute effectively to patents, reports, and publications of scientific findings

Education Requirements

Ph.D. in Organic Chemistry with 3+ years oligonucleotide chemistry experience

Skills and Experiences

• Demonstrated experience in the synthesis, purification, and characterizations of oligonucleotides
• Strong understanding and experience with various oligonucleotide chemistries
• Hands on experience with oligonucleotide synthesizers and purification apparatus
• Hands on experience with oligo-related analytical techniques
• Experience in gram-scale multiple step organic synthesis is a plus
• Experience in oligonucleotide conjugation is a plus
• Experience in biopharma/biotech is a plus
• Ability to collaborate with interdisciplinary team with excellent written and oral communication skills
• Ability to multi‐task to meet research and development goals
• Drive to solve problems and ability to work independently

Position Summary

Entrada is seeking a highly motivated, proactive, collaborative, and innovative scientist with a background in bioorganic chemistry to join Entrada’s discovery efforts. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists on applying cutting-edge peptide and oligonucleotide chemistry to develop leads for compelling biological targets. Specifically, the candidate will design and execute medicinal chemistry studies and use proprietary display technology to develop next-generation constrained peptidomimetics. The candidate will contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Design and execute the synthesis, purification, and characterizations of peptides and oligonucleotides development candidates
• Design and execute the synthesis and characterizations of peptide and oligonucleotide library for SAR studies
• Work with other team members to implement assays for ascertaining target engagement, validating biological activity in vitro (e.g. alphascreen, TR-FRET, fluorescence polarization, cell-based functional assay)
• Manage, organize, and critically evaluate data independently.
• Champion innovation and new initiatives in discovery programs by proactively reviewing literature and other advancements
• Interpret and present research data and findings in internal and external scientific meetings and conferences
• Contribute effectively to patents, reports, and publications of scientific findings

Education Requirements

• Ph.D. in organic chemistry, chemical biology, medicinal chemistry, bioorganic chemistry, or a relevant scientific discipline

Skills and Experiences

• Demonstrated experience in the design, synthesis, and purification of peptides and oligonucleotides
• Skilled with solid-phase peptide synthesizers, method optimization and conjugations
• Demonstrated experience with HPLC, LC-MS systems and other analytical instruments
• Hands-on experience with oligonucleotide synthesizers is a plus
• Hands-on experience with flow-based solid phase synthesis is a plus
• Experience with hit optimization, SAR study, target engagement and other medicinal chemistry principals is a plus
• Ability to collaborate with interdisciplinary team with excellent written and oral communication skills
• Ability to learn fast, grasp the “essence”, and multi-task to meet research and development goals
• Drive to solve problems and ability to work independently

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

We currently have an exciting opportunity available to join the Fermentation group at Entrada Therapeutics. We are seeking a highly motivated, collaborative, and innovative Sr. Research Associate with a background in fermentation/cell culture. The candidate will be responsible for the designing and execution of small- and medium-scale microbial (E. coli, Pichia pastoris) fermentations to support the discovery and development of Entrada’s biologics pipeline. The candidate will work with a world-class, multidisciplinary team of drug discovery and development scientists to evaluate new clinical candidates and develop new processes. Specifically, the candidate will focus on the improvements of fermentation and high throughput screening workflow to produce recombinant therapeutic proteins using microbial expression systems. The candidate will also contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Plan the fermentation and primary recovery experiments according to objective priority by interacting with various departments within Entrada
• Independently designs and troubleshoots fermentation and primary recovery experiments
• Set-up, inoculate, and operate meticulously shake flasks, small- and medium-scale yeast and bacterial fermentations
• Perform primary recovery such as cell harvest, homogenization, inclusion body wash, solubilization, and refolding
• Run SDS-PAGE gels, HPLC to monitor metabolite levels and protein expression
• Record experimental details and observations in the laboratory notebooks
• Present data and research findings in internal team meetings

Qualifications

• Ph.D./MS/BS in Biochemical Engineering, Biotechnology, Biochemistry or related fields
• Ph.D. or MS with 2+ years or BS with 4+ years of experience related to fermentation process development of recombinant proteins
• Experience working with E. coli, Pichia pastoris for expression of recombinant proteins
• Experience with primary recovery operations such as centrifugation, homogenization, solubilization, refolding (a plus)
• Knowledge and use of molecular biology and biochemistry techniques
• Strong understanding of bioreactor design and transport phenomena as it relates to bioprocess scale-up and applied control theory
• Desire to work in an innovative and collaborative environment pursuing high reward science

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for a Pharmacology/In Vivo Biology Senior Scientist to drive the Company’s intracellular biologics discovery programs. This individual will work closely with the Discovery Group and other functions to progress programs from target identification to preclinical proof-of-concept. The successful candidate is expected to be involved in all aspects of translational science, leading efforts for developing cell-based assays for candidate optimization, drug candidate validation and overseeing pharmacology studies for PK/PD and preclinical proof-of-concept.

The successful candidate will also provide biology insights on new targets and therapeutic hypotheses to guide drug discovery efforts and translation into the clinic. This individual is expected to directly lead from the bench and work with research associates and CROs to perform experiments and biological research. As part of the Biology Group, the Biology Senior Scientist has the opportunity to contribute broadly in advancing validated targets into the drug discovery pipeline. Title will be commensurate with experience.

Job Responsibilities

• Investigate and validate novel biology of interested targets
• Collaborate with Discovery Group to generate mechanistic proof-of-concept and contribute to selecting novel therapeutic modalities that include antibody, peptides and oligonucleotides
• Experience in PD biomarker identification and development for intracellular/extracellular targets
• Design, conduct and manage in vitro and in vivo experiments to evaluate mechanistic, , ADME and PK profiles of developmental candidates
• Experience in oligo nucleotide mediated delivery for neuromuscular targets
• Utilize internal and external resources to execute pharmacology studies and preclinical translational studies relevant for neurological disorders
• Engage key opinion leaders and collaborators to drive program to clinical development
• Mentor junior members of Discovery Biology Group
• Present data and research findings in internal team meetings, review meetings and external conferences

Qualifications

• Ph.D. or equivalent in neuromuscular biology or relevant field with strong publication record
• More than 5 years of industry experience preferably in rare genetic diseases
• Demonstrated understanding of in vitro and in vivo mechanistic and functional studies using oligonucleotide mediated delivery
• Experience working with multiple mammalian cell culture systems, including cell lines ,and primary cell cultures
• Hands-on experience in technologies including ELISA, immunohistochemistry, immunofluorescence, in-situ hybridization, western blotting, flow cytometry and qPCR
• Proven ability to work independently, solve problems and initiate new ideas
• Strong communication skill and ability to work effectively across functions
• “Hands-on” mentality in moving projects forward
• Desire to work in an innovative and collaborative environment pursuing high reward science

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for a highly motivated, collaborative and innovative Principal Scientist, specializing in pharmacokinetics and pharmacology to join the Company’s intracellular biologics discovery and development efforts.

The candidate will work with a world-class, multidisciplinary team of drug discovery scientists and lead the in vitro and in vivo ADME, PK, PD and toxicokinetic efforts. Specifically, the candidate will design, manage and execute in vitro and in vivo programs both inhouse and at contract research organizations. The candidate will also be responsible for analyzing, interpreting and modeling DMPK and PK/PD data. The candidate will contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Design, manage and execute in vitro, and in vivo experiments to evaluate the ADME and PK profiles as well as toxicokinetics and immunogenicity of developmental intracellular biologics candidates both in-house and at contract research organizations
• Develop and execute assays for measuring drug concentration, target engagement and pharmacodynamic effects in vitro and in vivo
• Champion innovation and new initiatives for discovery programs by proactively reviewing literature and other advancements in disease area biology
• Interpret and present research data and findings in internal and external scientific meetings and conferences
• Contribute effectively to patents, reports and publications of scientific findings

Qualifications

• Ph.D. in pharmaceutics, pharmacology or a relevant scientific discipline • A minimum of 8 years’ experience in pharmacokinetics and pharmacology
• Demonstrated knowledge of ADME, PK/PD and bioanalytical development of protein therapeutics/biologics
• Deep understanding of translational biology
• Experience in bioanalytical techniques including assays for immunogenicity, in vitro and in vivo toxicology and histopathology preferred
• Experience in assay development for target engagement and biomarker discovery preferred Experience in contributing DMPK and pharmacology sections of regulatory submissions preferred
• Ability to collaborate in an interdisciplinary team setting with excellent written and oral communication skills
• Ability to multi-task to meet research and development goals
• Drive to solve problems and ability to work independently Compensation is competitive and commensurate with experience.