Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is seeking a highly motivated, collaborative and innovative high-throughput Process Engineer specializing in the downstream processing of recombinant proteins/antibodies. The successful candidate will be responsible for purification process development, scale-up and tech transfer activities for multiple early-stage (first-in-human) and late-stage clinical programs.

Job Responsibilities

• Design and execute studies to develop, optimize and scale-up downstream unit operations including harvest/clarification, chromatography, UF/DF, conjugation, formulation and fill/finish 
• Play a key role in high throughput platform process development for evaluation and implementation of novel concepts
• Participate in cross-functional process development and discovery teams to support robust CMC documentation
•  Write protocols and operating procedures leading to tech transfer. Build strong working relationships with external CMOs and be the technical expert responsible for effective tech transfer
•  Interpret and present research/development data and findings in internal meetings
• Contribute effectively to patents, reports, and publications of scientific findings

Qualifications

• Ph.D. (0+years) / M.S. (2+years) / B.S. (4+ years) in Biochemical Engineering/Biotechnology/Biochemistry or related fields with relevant experience in downstream process development of biologics
• Hands-on experience with AKTA, UNICORN, TFF, HPLC, and TECAN/liquid handlers is required
• Working knowledge of DOE, QbD, and the ability to critically analyze data using statistical tools
• Experience in tech transfer to internal manufacturing or CDMOs is preferred
• Ability to multi-task while remaining organized to meet research and development goals
• Ability to collaborate with an interdisciplinary team with excellent written and oral communication skills
• Drive to solve problems and ability to work independently
• Ability to work with junior scientists to facilitate troubleshooting activities is desired

Entrada's Mission

Entrada Therapeutics’ mission is to treat devastating diseases through the intracellular delivery of biologics.

Compensation is competitive and commensurate with experience. Competitive health, dental and vision coverage. Life insurance and short-term and long-term disability insurance provided. Free monthly subway pass, subsidized parking or subsidized monthly commuter rail pass. 

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is seeking a highly motivated, collaborative and innovative Scientist/Sr. Scientist specializing in oligonucleotide therapeutics, including but not limited to antisense oligonucleotides and small interfering ribonucleic acids. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists on applying Entrada’s cutting-edge drug delivery platform to develop oligonucleotide-based pipelines for treating some of the most challenging diseases. Specifically, the candidate will design, prepare and characterize oligonucleotide constructs, and develop analytical assays for functional validation both in vitro and in vivo. The candidate will contribute to scientific publications and regulatory submissions as required. 

Job Responsibilities

• Design and optimize oligonucleotide sequences for DNA and mRNA targets 
• Prepare, optimize and purify oligonucleotides-peptide conjugates with different chemical strategies 
• Analytical characterization of the synthesized oligonucleotides and oligonucleotidespeptide conjugates 
• Develop and execute cell-based assays for validating biological activity of oligonucleotides-peptide conjugates in vitro
• Process and analyze samples from in vivo studies (e.g. qPCR, ELISA, Western blotting, confocal microscopy, HPLC)
•  Prepare SOPs and other reports/documents for assay development and validation 
•  Interpret and present research data and findings in internal and external scientific meetings and conferences 
•  Contribute effectively to patents, reports, and publications of scientific findings

Qualifications

Education Requirements

Ph.D. in chemistry, biochemistry, or related field

Skills and Experiences

• Demonstrated experience in design and validation of oligonucleotide-based drug delivery systems, such as antisense oligonucleotides and siRNAs 
• Demonstrated experience in conjugation and purification of oligonucleotides with different methods 
• Demonstrated experience in cell-based screening assays, such as qPCR, ELISA, luciferase, fluorescence and Western blotting  Expertise in cell culture including working with complex cell types preferred 
•  Experience with in vivo pharmacology preferred 
•  Experience with LC-MS systems and other bioanalytical methods 
• Ability to collaborate with interdisciplinary team with excellent written and oral communication skills
• Ability to multi-task to meet research and development goals 
• Drive to solve problems and ability to work independently

Entrada's Mission

Entrada Therapeutics’ mission is to treat devastating diseases through the intracellular delivery of biologics.

Compensation is competitive and commensurate with experience. Competitive health, dental and vision coverage. Life insurance and short-term and long-term disability insurance provided. Free monthly subway pass, subsidized parking or subsidized monthly commuter rail pass. 

Company Overview

Entrada Therapeutics is a biotechnology company dedicated to transforming the treatment of devastating diseases through the intracellular delivery of biologics. Entrada’s technology enables the efficient intracellular delivery of proteins, peptides and nucleic acids, thus allowing for the development of programs across several intracellular target classes. The Company’s novel approach addresses current challenges associated with both large and small molecule therapeutics and represents a fundamental advancement in the delivery of molecules into the cytosol.

Entrada is comprised of experts and leaders in both biologics development and the rare disease space. In December 2018, Entrada closed a $59 million Series A from 5AM Ventures, MPM Capital, Roche Venture Fund, MRL Ventures Fund and Agent Capital.

Position Summary

Entrada is hiring for a highly motivated, collaborative and innovative Principal Scientist, specializing in pharmacokinetics and pharmacology to join the Company’s intracellular biologics discovery and development efforts. The candidate will work with a world-class, multidisciplinary team of drug discovery scientists and lead the in vitro and in vivo ADME, PK, PD and toxicokinetic efforts. Specifically, the candidate will design, manage and execute in vitro and in vivo programs both inhouse and at contract research organizations. The candidate will also be responsible for analyzing, interpreting and modeling DMPK and PK/PD data. The candidate will contribute to scientific publications and regulatory submissions as required.

Job Responsibilities

• Design, manage and execute in vitro, and in vivo experiments to evaluate the ADME and PK profiles as well as toxicokinetics and immunogenicity of developmental intracellular biologics candidates both in-house and at contract research organizations 
•  Develop and execute assays for measuring drug concentration, target engagement and pharmacodynamic effects in vitro and in vivo 
• Champion innovation and new initiatives for discovery programs by proactively reviewing literature and other advancements in disease area biology 
• Interpret and present research data and findings in internal and external scientific meetings and conferences
•  Contribute effectively to patents, reports and publications of scientific findings

Qualifications

• Ph.D. in pharmaceutics, pharmacology or a relevant scientific discipline • A minimum of 8 years’ experience in pharmacokinetics and pharmacology 
• Demonstrated knowledge of ADME, PK/PD and bioanalytical development of protein therapeutics/biologics 
• Deep understanding of translational biology
• Experience in bioanalytical techniques including assays for immunogenicity, in vitro and in vivo toxicology and histopathology preferred
• Experience in assay development for target engagement and biomarker discovery preferred Experience in contributing DMPK and pharmacology sections of regulatory submissions preferred
•  Ability to collaborate in an interdisciplinary team setting with excellent written and oral communication skills 
•  Ability to multi-task to meet research and development goals
•  Drive to solve problems and ability to work independently Compensation is competitive and commensurate with experience. 

Entrada's Mission

Entrada Therapeutics’ mission is to treat devastating diseases through the intracellular delivery of biologics.

Compensation is competitive and commensurate with experience. Competitive health, dental and vision coverage. Life insurance and short-term and long-term disability insurance provided. Covered monthly subway pass, subsidized parking or subsidized monthly commuter rail pass.